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Microbial "Contaminants" in Plant Tissue Cultures: Solutions and Opportunities 1996-2003

Volume 8 of Recent Advances in Plant Tissue Culture

edited by E.B. Herman

Agritech Consultants, 2004. 122 pages. Price: $129.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

Microbial "Contaminants" in Plant Tissue Cultures: Solutions and Opportunities 1996-2003 is the eighth in a series, based on articles published in Agricell Report, that reports recent developments in plant tissue culture. Unlike most plant tissue culture books, which have long publication delays, the Recent Advances in Plant Tissue Culture series is updated within one month of the book’s publication date.

In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full addresses of cited authors is included. The directory includes e-mail addresses when available. The book also includes a list of meetings and symposia in which contamination-related papers were presented, a taxonomic index, and an index of antimicrobial, antifungal, antiviral and acaricidal agents.

Partial Contents: CHAPTER 1. PLANT TISSUE CULTURE CONTAMINANTS - Sources of contamination. Detection, identification, and characterization of microbial contaminants. Latent contamination. CHAPTER 2. METHODS FOR ELIMINATING CONTAMINANTS - Disinfestation of explants and elimination of microbial infections: Wasp-induced gall tissue as a source of explant material, NaDCC, Antibiotics, Fungicides, PPM, Benomyl + NaDCC, Ribosome inactivating protein, Use of tea strainer or tea bags, Thermotherapy, Acetylsalicylic acid, Ultrasound, Hot water, Chlorine-containing sterilants, Essential oils, Bag-spray-graft technique, Meristem reversion, Electrotherapy, Lysozyme, ACZP, Dry heat, Silver nitrate, Stepwise disinfestatation techniques, Comparison of disinfestation techniques, Seed disinfestation. Control of Arthropod contamination. Control of Nematode contamination. Control of virus contamination: Stem-disc dome culture, Plant extract virus inhibitor, CRyopreservation, Electrotherapy, Shoot tip culture, Ribavirin. Elimination of microbial contaminants from culture media: Microwave sterilization, Sodium hypochlorite or sodium dichloroisocyanurate, Comparison of sterilization procedures. Effects of Antibiotics on tissue cultures. CHAPTER 3. NON-AXENIC PLANT TISSUE CULTURE - Micropropagation under non-sterile conditions: "Microponic" production of plants, Large-scale photoautotrophic micropropagation, Use of thermo-responsive hydro-gel, Use of chlorine in tissue culture medium, non-sterile culture of ginseng, Toleration of contaminants in micropropagation, Non-sterile culture of hairy roots. Coculture with microorganisms e.g., "bacterization": Promotion of growth, regeneration and rooting, Prevention of hyperhydricity, Stimulation of secondary metabolite production and release, Induction of resistance to fungal infection, Resistance to high temperature, Increase in plantlet survival, biological hardening, control of browning. Mycorrhization. Agrobacterium rhizogenes and rol genes: Use of rol genes in micropropagation, Mechanisms of action of rooting enhancement by A. rhizogenes, Hairy root cultures, Control of secondary metabolite synthesis by hairy root rol genes, Secondary metabolite synthesis by hairy root cultures. Nitrogen fixation. Use of microbial products in plant tissue cultures: Bacterial product substitutes for IAA in micropropagation medium, Fungal culture filtrate induces potato microtuberization. CHAPTER 4. PRESERVATION OF VIRUSES VIA PLANT TISSUE CULTURE

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