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"Regeneration and Micropropagation: Techniques, Systems and Media 1991-1995

Volume 3 of Recent Advances in Plant Tissue Culture

by Edwin B. Herman, Editor, Agricell Report

Recent Advances in Plant Tissue Culture: Regeneration and Micropropagation: Techniques, Systems and Media 1991-1995 is the third in a series, based on articles published in Agricell Report, that reports the previous 3-4 years’ developments in a specific area of plant tissue culture.

In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full addresses of cited authors is included. The book also includes a list of recent symposia and a taxonomic index.

Partial Contents: 1. New techniques. Etiolation. Direct seed culture. Somatic embryo culture, maturation, conversion, concentration and purification. Preparation of explants — decapitation, squashing & homogenization, new cutting techniques. Ventilation. Rapid opening & closing device. Avoiding condensation. Control of browning. Lighting. Photoautotrophic culture. Media - measuring & reducing phytotoxicity, controlling pH, conditioned media & nurse cultures, inexpensive media. Controlling regeneration pathways. Image analysis measurement of growth & injury. 2. Systems. Solid-liquid sequential media. Bioreactors — temporary immersion, mist, fixed-bed, liquid culture. Batch cultures. Continuous flow systems. Oxygenation. Supports. Control of phytohormone concentrations. Automated sytems. Artificial seed coating system. 3. Enhancement of Regeneration and Growth. Chemical, physical and biological factors. Mineral oil overlay. Statistical optimization of embryogenesis. Genetic control of regeneration. Selection for regenerability. Uniform plants from genetically variable cultures. Direct regeneration. Maintaining & restoring embryogenic capacity. 4. Micropropagation. Techniques — micrografting, in vitro rooting, mycorrhizal fungi, vernalization, rejuvenation. Control of shoot-tip necrosis. Hyperhydricity (vitrification) and acclimatization. Micropropagation of seedless watermelon, Citrus, potato, spruce, grapevine, orchids. Evaluation of plant production. Storage & shipment of micropropagules. Plant improvement via micropropagation. Field performance of micropropagated plants. 5. Novel applications of plant tissue cultures. Pseudo tomatoes. Detection of water pollutants. Bioremediation. Nitrogen fixation by non-legumes — a new approach. Screening for biological activity. Preventing allergies. Biosensors. Tissue culture-mediated sex shifts.

Publication: 1995, 183 pp. Price: US$ 129.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

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