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"Regeneration, Micropropagation and Media 1988-1991"

Volume 1 of Recent Advances in Plant Tissue Culture

by Edwin B. Herman, Editor, Agricell Report

"Recent Advances in Plant Tissue Culture: Regeneration, Micropropagation and Media 1988-1991" is the first in a series, based on articles published in Agricell Report, that reports the previous three to four years’ developments in a specific area of plant tissue culture.

In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full addresses of cited authors is included. The book also includes a list of meetings and symposia, with references.

Partial contents: 1. Regeneration from in vitro cultures. Regeneration enhancing effects of: stresses (heat, salt, plant pathogens, electrical stimulation, gamma irradiation, growth inhibitors), ethylene and ethylene antagonists (silver nitrate, aspirin), ventilation, phenolics, calcium, cobalt, vitamin C, antibiotics, amino acids, carbohydrates, gelling agents, glycoproteins, growth regulators, activated charcoal, light quality, cell wall components, medium conditioning substances, cocultivation with microorganisms, verticillium filtrate, pretreatment of source material. Genetic control of differentiation. Selection and breeding for regenerability. Long-term regeneration. Possible mechanisms of regeneration enhancement. . 2. Early predictors of regenerability. Chemical markers. Visual selection. Staining. Membrane fluidity. Rapid biochemical tests. 3. Rooting of micropropagated plants. Influence of Agrobacterium rhizogenes, antioxidants, pH, container type, light and temperature. Rooting in non-sterile sand. Prevention of shoot-tip necrosis. 4. Transition of plants from in vitro cultures to establishment in vivo. Prevention of water loss. Control of vitrification. Effects of light intensity and relative humidity. Role of inositol. 5. Growth and quality of micropropagated plants. Effects of mycorrhizae. Use of Trichoderma. Control of phenolic oxidation. Prevention of shoot-tip necrosis. Effect of mother plant environment. 6. Plant tissue culture media. Sterilization, Preventing degradation of media components. Feeder cultures and conditioned media. Control of medium pH. Agar replacements. Autoclave-induced toxicity. 7. Novel tissue culture systems and techniques. Semipermeable membrane system. Inexpensive alternative to transfer of cultures. Submerged culture system. Shake cultures. Novel support systems. Methods for increasing oxygenation. Carbon dioxide enrichment. Cocultivation with microorganisms. Automation and scale-up. 8. Hazards to plant tissue cultures. Phytotoxicity from media and culture vessels. Air pollutants.

Publication: 1991, 103 pp. Softcover. Price: US$ 129.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

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