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Volume 18 of Recent Advances in Plant Tissue Culture

by Edwin B. Herman, Editor, Agricell Report

"PLANT MICROPROPAGATION: 2010-2013" is the eighteenth in a series, based on articles published in Agricell Report, that reports recent developments in plant tissue culture. The Recent Advances in Plant Tissue Culture series is updated within one month of the book's publication date. In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full email and postal addresses is included. The book also includes a plant taxonomic index.



I. Liquid System for Micropropagation of Licorice

II. One-step Micropropagation in a Mist Bioreactor

III. Temporary Immersion Systems

A. Thin-Film Intermittent Wetting System B. Magnetic Field Systems C. Fingerling Culture D. Twin-flask Temporary Immersion Bioreactor E. Improved Siraitia grosvenorii Micropropagation F. Control of Hyperhydricity G. Scale-up of Temporary Immersion Micropropagation

IV. Vertical In Vitro Culture on Hanging Strips

V. Comparison of Liquid and Solid Culture Systems

VI. Combined Semisolid/Temporary Immersion System

VII. Combined Heterotrophic and Photoautotrophic Culture

VIII. Combination of In Vitro Culture and Hydroponics

IX. Aeroponic Potato Seed Tuber Production

X. Industrial Scale Plant Production in an Artificial Plant Ovary

XI. Comparison of Three Micropropagation Systems

XII. Eight In Vitro Systems for Date Palm Micropropagation


I. New Methods for Developing Micropropagation Media

A. Chemical Analysis of Seeds and Explants B. Data Mining C. Response Surface Methodology

II. Comparison of Supports for Plant Regeneration

III. Preculture with Diazuron

IV. Alternative Gelling Agents

V. Reproductive Structures as Explant Sources

VI. Use of Gamma Irradiation

VII. Improved Medium for Maize Micropropagation

VIII. Meta-topolins Enhance Micropropagation

IX. Micropropagation Using Biofertilization

A. Cyanobacterial Liquid Medium B. Cyanobacterial Extracts C. Coculture with Bacteria or Mycorrhizal Fungi

X. Use of Quiclorac

XI Methylglyoxal Promotes Differentiation

XII. Enhancement of In Vitro Maturation and Germination

A. Lauric Acid B. DMSO C. Electromagnetic Radiation D. NaOCl E. Heat Pretreatment F. Alar G. Activated Charcoal and/or Vitamins H. Fruit Juices I. Effects of Culture Media Water Relations J. Factors Affecting In Vitro Seed Germination K. Comparison of Embryo Rescue Techniques

XIII. Improved Rooting of Micropropagules

A. Ex vitro Rooting B. Bacteria Can Replace IBA for Rooting C. Organic and Chemical Fertilizers D. Heat Stresses E. Aloe Gel F. Effects of Light Quality G. Effects of Growth Inhibitors H. Effect of IBA and Electro-pulses I. In Vitro Rooting Substrates J. Phenolics K. Double Agar Layer Treatment L. Abiotic Stresses M. Additive Effects of Auxins and Copper

XIV. Evaluating Photosynthetic Properties of Micropropagules

XV. Promotive Effects of Hypobaric Growth Conditions

XVI. PBU Enhances Micropropagation 51

XVII. Micropropagation Enhancement by Antibiotics

XVIII. Self-Pollination Improves the Micropropagation Capacity

XIX. Alternate Pathways for Regeneration

XX. Long-term Sustained Micropropagation of Grasses

XXI. BTH and OTC Stimulate In Vitro Growth of Prunus

XXII. Sorbitol Enhances Regeneration of Upland Rice

XXIII. Micropropagation with New Diphenylurea Derivatives

XXIV. 24-Epibrassinolide Enhances Micropropagation

XXV. Salicylic Acid Improves Micropropagation

XXVI. Prevention of Shoot Tip Necrosis

XXVII. Use of Mature Embryos and Dicamba for Regeneration

XXVIII.Effects of Adenine Sulfate, Diphenylurea and Phlorglucinol

XXVIX. Micropropagation Enhancement by Smoke-derived Compounds

XXX. Betaine Improves Micropropagation of Tea

XXXI. Benzothiadiazole Enhances Regeneration

XXXII. Effects of Non-ionic Surfactants on Micropropagation

XXXIII. A Universal Method for Regeneration from Protoplasts

XXXIV. Low-cost Medium Supplements

XXXV. Effects of Light Quality

XXXVI. Direct Plant Regeneration from Pre-plasmolysed Explants

XXXVII.. Enhanced Somatic Embryogenesis Via Gene Expression

XXXVIII. Production of Haploid and Homozygous Diploid Plants

A. Genome Elimination B. Microspore and Pollen Culture XXXIX. Comparison of Haploid with Doubled Haploid Micropropagation

XL. Exogenous Polyamines Enhance Somatic Embryogenesis


I. Acclimatization of In Vitro Plantlets

A. Enhancement by Meta-topolin B. Enhancement by Bacterization C. Pretreatment of Explants D. Endosymbiotic Root Fungus as a “Biopriming” Agent E. Substrates for Acclimatization F. Enhancement by Exogenous Silicon G. Hydroponics for Acclimatizing Micropropagules H. Hyperhydricity Control in Apple , Watermelon and Vaccinium I. Seedling and Micropropagule Development Compared J. Optimization of Potting Mixture K. Effect of Irradiance Levels

II. Storage of Micropropagules

A. Trehalose Extends In Vitro Storage Period

III. Genetic Stability of Micropropagated Turmeric


I. Bananas

A. Bacterization B. Cost-Effective Methods for Banana Micropropagation C. Mycorrhization D. Smoke-water and Vermicompost Leachate E. Control of Browning in Banana Meristem Bud Clump Cultures

II Orchids

A. Effects of Chitosan on Orchid Micropropagation B. Reducing the Cost of Orchid Culture Medium C. Microwave “Sterilization” of Orchid Culture Medium D. One-Step Technique for Hardening Orchid Micropropagules E. Improved Acclimatization of Micropropagated Orchid F. Effects of Light Quality on In Vitro Orchid Culture G. Effects of Light and Supplements H. Improved Cymbidium Regeneration I. Coir Fibers: An Inexpensive Substitute for Agar J. Optimal Conditions for Production of Synthetic Orchid Seeds K. Embryo and Protoplast Isolation from Orchid Seeds L. In Vitro Seed Germination M. Seed Baiting Technique N. Effects of Organic Additives and Carbohydrate Sources O. Brick Pieces as Supporting Material for Orchid Culture P. Smoke-saturated Water Enhances Orchid Micropropagation Q. Induction of Petal-bearing Somatic Embryos from Orchid Callus R. A New Growth Regulator for Orchid Micropropagation S. Topolins in Pelargonium Micropropagation T. Effect of Orchid Cutting Methods on Shoot Multiplication U. Ultrasound Enhances Conversion of PLBs to Shoots V. Effect of Silicon on Orchid Micropropagation W. Urea as a Nitrogen Source X Endophytic Bacteria Promote Growth 108

III. Potatoes

A. In Vitro Multiplication of Potato in Plastic Rolls B. Effect of Supporting Material and Nutrient Supply Cycle C. Antibacterial Agent Affects Microtuberization and Growth D. Effects of Salicylic Acid E. Soil Solarization Improves Growth of In Vitro-cultured Tubers F. Inorganic Supporting Materials for Phototrophic Culture G. 3-Methoxy benzamide Enhances Potato Micropropagation


I. In Vitro Flowering

II. In Vitro Selection

A. Salt-Tolerant Plants Via Plant Tissue Culture Selection B. Selecting Ecotypes for Conservation and Restoration

III. Eliminating Potato Potyviruses via Electrotherapy

IV. Low-cost Commercial Production of Virus-free Sweet Potato

V. Silver Nanoparticles Synthesized by In Vitro-derived Plants

VI. Production of Bioactive Compounds


I. Aerated Liquid Priming of Conifer Somatic Embryos

II. Abscisic Acid Promotes Development of Somatic Embryos

III. Improving Germination of Embryos from Synthetic Seeds



Publication: November, 2013. 136 pages. Spiralbound. Price: $139.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

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