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"Media and Techniques for Growth, Regeneration and Storage 2005-2008"

Volume 12 of Recent Advances in Plant Tissue Culture

by Edwin B. Herman, Editor, Agricell Report

"Media and Techniques for Growth, Regeneration and Storage: 2005-2008" is the twelfth in a series, based on articles published in Agricell Report, that reports recent developments in plant tissue culture. The Recent Advances in Plant Tissue Culture series is updated within one month of the book’s publication date. None of the material in this book has been published in previous volumes of the series. In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full email and postal addresses is included. The book also includes a list of meetings and symposia and a taxonomic index.



I. Designing Plant Tissue Culture Media
A. Medium Ion Solution Calculator
B. Improved Media for Conifer Somatic Embryogenesis
C. Culture Media Based on Chemical Analyses of Intact Plants
D. Faster Cell and Callus Growth Using Organic Nitrogen and Phosphorus
E. Liquid Overlays Improve Embryogenic Tissue Initiation

II. Water and Carbon Sources
A. Tap water and Commercial-grade Sugar
B. Date Palm Syrup Replaces Sugar as Carbon Source
C. Use of Commercial-Grade Sugar and Isubgol

III. Solidifying Agents and Explant Supports
A. Locust Bean Gum
B. Galactomannans (Cassia and Guar Gum)
C. Xanthan Gum
D. Coir
E. Cassava Starch
F. Comparison of Commercial Gelling Agents
G. Agar Impurities Affect Shoot Quality & Multiplication
H. Comparison of Explant Support systems

IV. Analyzing Plant Culture Media
A. Determining 2,4-D Levels in Media Containing Activated Charcoal

V. Physical Properties of Tissue Culture Vessels


I. Coculture of Explants
A. Coculturing Two Cucumber Cultivars: Effect on Callus Growth
B. Cocultivation of Shoots Improves In Vitro Rooting

II. Lighting Systems
A. Advanced Sensors and Lighting for In Vitro Cultures

III. Plant Cell Culture Techniques
A. New Tools for Assessing Cell Viability
B. In Vitro Culture of Plant Cell Organelles
C. Protoplast Culture
D. Protoplast Fusion
E. Culture of Isolated Egg Cells and Zygotes
F. Fusion of Gametes In Vitro
G. A Fine, Rapidly Proliferating Rice Cell Suspension Culture
H. Culture of Plant Cells in Liquid Media Without Agitation
I. Bioreactor Systems for Cell Culture
J. Cytometric and Visual Selection of Stable and Regenerable Cell Lines
K. Pollen Culture
L. Culture of Isolated Microspores

IV. Organ Culture Techniques
A. Seed Culture
B. Ovule Culture
C. Hairy Root Cultures
D. Anther Culture
E. Embryo Culture
F. Applications of Embryo Culture

V. Control of Condensation on Petri Dish Lids During Tissue Culture

VI. Control of Explant Browning

VII. Long-term Maintenance of Grapevine Embryogenic Cultures

VIII. Relationship Between DNA Methylation and In Vitro Plant Regeneration

IX. Early Prediction of Morphogenetic Capacity
A. Physiological parameters
B. Proline a Biochemical Indicator
C. In Vitro Height
D. Pyrolysis Mass Spectroscopy
E. Mathematical Model for Optimal Plant Cell Growth Conditions

X. Somaclonal Variation
A. Regeneration of Bamboo Inflorescences Without Stamens
B Crop Failure Caused by Somaclonal Variation

XI. Manipulation of Morphogenic Pathways

XII. Chromosome Doubling
A. In Vitro Colchicine Treatment for New Cultivar Development
B. Improved Efficiency of Canola Doubled-Haploid Production
C. Comparison of Antimitotic Agents for Chromosome Doubling

XIII. Aluminum Versus Paper Ventilation Closures

XIV. Toxicity of Silicon Tubing to In Vitro-cultured Plant Cells

XV. In Vitro Grafting

XVI. A Free Database for Managing Plant Research


I Chemical Factors: Iron Chelates, Copper, Helium, Boron, Boron and Methionine, Oxygen, Nitric Oxide, Calcium, Nitrogen, Deuterium-depleted Water, Propylene Glycol, Polysaccharides, Polyvinylpyrrolidone, Organic Acids and Vitamins, Carbohydrates, Pectimorph, Proteoglycans, Amino Acids, Butenolides, Methyl glyoxal, Triacontanol, Plant Growth Regulators, Brassinosteroids, Hemoglobin, Flurprimidol, BSO, Humic Substance Extracts, Lignosulfates,Peptone

II. Physical Factors: pH, Light (Light Quality, Intermittent Flashing Light, Laser Irradiation, Infrared Irradiation), Electric Current, pH, Gamma Irradiation, Magnetic Field, Sound, Ultrasound, Plasmolysis

III. Biological Factors: Trichome Secretions, Carrot Embryogenic Callus Extract and Salicylic Acid, Plant Extracts


I. In Vitro Collection

II In Vitro Preservation
A. Low-temperature Storage
B. Cryopreservation
C. Encapsulation
D. Encapsulation and Synthetic Seeds
1. Two-Layer Artificial Seeds
2. Novel Cell and Tissue Encapsulation Membrane Possibilities
3. Encapsulation Increases Low-Temperature Storability of Somatic Embryos
4. Synthetic Seeds of Mulberry and Cymbidium
5. Plant Regeneration from Synthetic Seeds

III. Digital-image Evaluation System for Health of In Vitro-stored Plant Germplasm


I. Truffle Production using Hairy Root Cultures

II. Rapid In Vitro Evaluation of Plant Responses to Organic Waste Products

III. Using Callus Traits to Select for Improved Sugarcane Genotypes

IV. In Vitro Seed Germination and Mycorrhization Yields Edible Mushrooms

V. Phytoremediation of Zinc by Black Nightshade Hairy Root Cultures

VI. Phytoremediation via Plant Tissue Culture and Biotechnology
A. Bioremediation of Lead and Chromium by Root Cultures
B. Enhanced Processing of Organomercury

VII In Vitro Screening for Potato Drought Resistance in the Field



Publication: March, 2008. 133 pp. Spiralbound. Price: $129.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

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