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"Microbial Contaminants in Plant Tissue Culture III 2003-2007"

Volume 11 of Recent Advances in Plant Tissue Culture

by Edwin B. Herman, Editor, Agricell Report

"Microbial Contaminants in Plant Tissue Culture III: 2003-2007" is the eleventh in a series, based on articles published in Agricell Report, that reports recent developments in plant tissue culture.

Unlike most plant tissue culture books, which have a long publication delay, the Recent Advances in Plant Tissue Culture series is updated within one month of the book’s publication date. None of the material in this book has been published in previous volumes of the series.

In order to make it easy for the reader to obtain further information on material covered in the book, a directory consisting of full addresses and fax numbers of cited authors is included. The directory includes e-mail addresses when available. The book also includes a list of meetings and symposia in which contaminant-related papers were presented, a taxonomic index, and an index of antimicrobial, antifungal, anti-insect, and antiviral agents.



I. Detection, Identification and Characterization of Microbial Contaminants, A. PCR-Based Approaches, B. Electronic Nose, C. Detecting Latent (Covert) Bacteria, D. Endogenous Bacteria, E. Phytoplasma Detection, II. Latent Contamination. A. Bacterial Survival in 5- to 6-year-old Tissue Cultures, B. Viral Survival in 16-year-old Tissue Cultures. III. Detection and Elimination of Viruses in Lily Explants


I. Sterilization Procedures. A. Filter Sterilization, B. Use of Chlorine Compounds, C. Effects of Chlorine Compounds on Explants, D. Microwave Sterilization. II. Disinfestation of Explants and Control of Culture Contamination, A. Formaldehyde Fumes Disinfest Orchid Seeds, B. Comparison of Three Compounds for Disinfestation, C. Production of Uncontaminated Explants, D. Control of Contamination During Papaya Micropropagation, E. Lysozyme, F. Antibiotics, 1. Meropenem and Moxalactam 2. Control of Contamination in Pyrethrum Callus Cultures, 3. Disinfesting Long-Term Micropropagated Plants, 4. Bavistin and Tetracyclin, 5. Bavistin and Trimethoprim, 6. A Novel Antibiotic, 7. Comparison of 34 Different Antibiotics, G Effects of Antibiotics on Tissue Cultures and Transformation, 1. Antibiotics and Bialaphos, 2. Penicillin Derivatives, 3. Paromomycin and Cryopreserved Cells, 4. Cefotaxime, 5. Stimulation of Morphogenesis & Reduced Necrosis, 6. Regeneration from Tea and Mothbean Explants, 7. Effects on Embryogenic and Organogenic Tissues, H. PPM, I. New Techniques, 1. Flame Disinfestation, 2. Use of Zerotol, Control of Vitropathic Contaminants, 4. Change of Exogenously Supplemented Hormone, 5. Use of Plant Extracts, Control of Latent Contamination. III. Comparison of Contamination Control Methods. IV. Selection of Uncontaminated Explants. V. “Clean Cultures” via In Vitro Collecting. VI. Control of Thrips Contamination. VII. Elimination of Mite and Fungal Contamination. VIII. Cleanup of Yeast Contaminants. IX. Elimination of Viruses, A. Cryopreservation, B. Antiviral Agents, C. A Novel Method for Eliminating Chrysanthemum Viroid, D. Electric Current and Ribavirin, E. Root Meristem Culture, F. Cytokinin Sequence Application, G. Comparison of Methods for Virus Elimination. X. Biosynthesis of Antibacterial Agents by In Vitro Plant Cultures, A. Antimicrobials from Rauvolfia and Physalis Cultures, B. Antimicrobials from Nigella Cultures, C. Agent Active Against Staphylococcal Biofilms


I. Coculture with Bacteria, A.Alcohol-Tolerant Contaminant Enhances Micropropagation, B. Promotion of In Vitro Root Growth at Low Temperature, C. Inhibition of Pathogen Action and Improved In Vitro Growth, D. Biotic Priming of Plant Tissue Cultures, E. Effect of Seed/Seedling Bacterization on Micropropagation, F. Bacterial Strain Mitigates Cucumber Root Exudate Autotoxicity, G. Methylobacterium Stimulates In Vitro Development, H. Bacillus Promotes Growth, Development and Survival, I. Biolistic Introduction of Nitrogen-fixing Bacteria, J. Bacterization Stimulates Isoflavone Yield by Root Cultures. II.Uses for Agrobacterium rhizogenes and rol Genes, A. Positive Effects of Agrobacterium rhizogenes Infection, 1. Enhanced Root Development, 2. Phenotypic Alterations, 3. Enhanced Regeneration, B. Effects of rol Genes, 1. Unique Plant Phenotypes, 2.Enhanced Embryogenesis, 3. Enhanced Production of Secondary Metabolites, III. Hairy-Root Cultures, A. High Phytoestrogen Levels from Shoot-Hairy Root Coculture, B. Circadian Rhythms in Hairy Root Cultures, C. Bioreactors for Hairy-Root Cultures, D. Truffle Production using Cistus incanus Hairy Root Cultures, E. Production and Release of Secondary Metabolites, 1. Betalain Pigments, 2. Camptothecin and 10-Hydrocamptothecin, 3. Scopolamine, 4. Opiates, 5. Puerarin, 6. Anti-Hepatitis Extract, 7. Artemisinin, 8. Solasodine, 9. Peroxidase, 10. Azadirachtin, F. Heat-Inducible Gene Expression. IV. Coculture with Microalgae and Cyanobacteria. V. Coculture with Fungi . A. Inoculant for In Vitro Mycorrhization of Plants, B. Effects of Mycorrhizal Fungal Inoculation on: 1. Artichoke, 2.Raspberry, 3. Lucomo, 4.Rhododendron, 5. Cherimoya, 6. Grapevine, 7. Orchids, 8. Scots Pine, B. Fusarium Inoculation, C. Rhizoctonium Inoculation, D. Association of Endophytic Fungi with Micropropagated Plants, E. Inoculation with Aspergillus ustus and Puccinia sp., F. Inoculation with Piriformospora indica. VI. Plant Virus Propagation via In Vitro Plant Culture


I. Cyanobacterial Compounds Promote Regeneration. II. Trichosanthin


Genetic Engineering by Non-Agrobacterium Bacteria. II. New Techniques for Agrobacterium-Mediated Transformation, A. Macerating Enzymes, B. Embryogenic Calli under Dehydration Stress, C. Centrifugation-Assisted Transformation, D. Liquid Culture, E.Matrix Attachment Regions, F. Use of Stem Thin Cell Layers, G. Use of Plasmolysis, H. Use of Scutellum-Derived Calli, I. Multineedle Technique, J. Half-Seed System, K. Meristem Wounding, L. Okadaic Acid or Trifluoperazine





Publication: June, 2007. 116 pp. Spiralbound. Price: $129.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

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