Methods and Protocols
Thorpe, Trevor A. and Yeung, Edward C.
1st Edition., 2011, XII, 380 p. 30 illus., Hardcover
Price: $169.00 (Shipping and handling: $8.00 for U.S. delivery, $25.00 elsewhere).
Part I: Culture of Zygotic Embryos: Fundamental Studies 1. Zygotic Embryo Culture: An Overview 2. In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture 3. Canola Zygotic Embryo Culture 4. Immature Seeds and Embryos of Medicago truncatula Cultured In vitro 5. In vitro Culture and Germination of Terrestrial Asian Orchid Seeds 6. In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos Part II: Application of Embryo Culture Techniques: Embryo Rescue 7. Immature Embryo Rescue and Culture 8. Chickpea Hybridization Using In vitro Techniques 9. Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture 10. Phaseolus Immature Embryo Rescue Technology 11. Wide Crossing in Lentil through Embryo Rescue 12. Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques Part III: Cryopreservation of Embryos 13. Cryopreservation of Embryos: An Overview 14. Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm 15. Cryopreservation of Zygotic Embryo Axes and Somatic Embryos of European Chestnut 16. Cryopreservation of Ilex Immature Zygotic Embryos Part IV: The Use of Zygotic Embryos as Explants for Somatic Embryogenesis and/or Organogenesis 17. The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview 18. Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos 19. Pine Somatic Embryogenesis Using Zygotic Embryos as Explants 20. Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies Part V: Transformation Using Zygotic Embryos as Explants 21. Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview 22. Genetic Transformation Using Maize Immature Zygotic Embryos 23. Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue 24. Agrobacterium tumefaciens-Mediated Genetic Transformation of Cereals Using Immature Embryos
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