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In vitro Culture of Higher Plants
4th Edition
byR.L.M. Pierik
Dept. of Horticulture, Agricultural University, Wageningen, the Netherlands
In Vitro Culture of Higher Plants presents a wide-ranging account of the techniques and applications, and has
primarily been written in response to practical problems. Special
attention has been paid to the educational aspects.
Typical methodological aspects are described in the first part:
laboratory set-up, composition and preparation of media, sterilization
of media and plant material, isolation and (sub)culture,
mechanization, the influence of plant and environmental factors on
growth and development, the transfer from test tube to soil, aids to
study. The question why in vitro culture is practised is covered in
the second part: embryo culture, germination of orchid seeds,
mericloning of orchids, production of disease-free plants, vegetative
propagation, somaclonal variation, test-tube fertilization, haploids,
genetic manipulation, other applications in phytopathology and plant
breeding, secondary metabolites.
Contents
1. Preface. 2. Introduction. 3. History.
4. Types of Cultures. 5. Laboratory Equipment. 6.
Preparation and Composition of Nutrient Media. 7. Closure of
Test Tubes and Flasks. 8. Care of Plant Material. 9.
Sterilization of Plant Material. 10. Isolation, Inoculation and
Subculturing. 11. Mechanization. 12. The Influence of
Plant Material on Growth and Development. 13. The Influence of
Physical Factors on Growth and Development. 14. The Transfer
from Nutrient Medium to Soil. 15. Aids to Study. 16.
Embryo Culture. 17. Germination of Orchid Seeds. 18.
Vegetative Propagation of Orchids. 19. Production of
Disease-Free Plants. 20. Vegetative Propagation. 21.
Somaclonal Variation. 22. Test Tube Fertilization. 23.
Production of Haploids. 24. Genetic Manipulation. 25.
Miscellaneous Applications. 26. In Vitro Cloning of Plants in
the Netherlands. 27. Index. 28. Literature Cited.
Kluwer Academic Publishers, Dordrecht
Softbound, ISBN 0-7923-4527-4
1999
$269.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).