"Introduction to Plant Tissue Culture"

Science Publishers, September 2002, 376 pages. $ 49.50 + shipping ($5.00, U.S. or $15.00 Elsewhere)

In this book author has combined his extensive research experience using plant tissue culture with his extensive knowledge of the needs of students for a subject that increasingly pervades most aspects of the plant sciences. Moreover, the book avoids the fragmentation often present in multi-authored compilations. Students need to have at hand a clearly written, well-documented text that puts into perspective the plant tissue culture requirements for particular applications, yet which also enables students to undertake experiments themselves with the minimum of additional guidance. Students will benefit greatly from this clearly presented and illustrated text, and from the analysis of the historical development of a subject with its beginnings in the 18th Century, in studies of callus formation associated with wound healing in trees, to its use in cell and molecular biological studies of plant genetic manipulations. This book is particularly well referenced, enabling the reader to follow up in greater detail points of interest, either theoretical or practical. Plant tissue culture, mainly because of the great number of interacting factors involved, tends to be largely empirical. In future years the applications of plant tissue culture in clonal propagation, plant breeding and plant genetic manipulation will increasingly depend on a better understanding of these interacting factors.

INTRODUCTION AND TECHNIQUES

Introductory History
Laboratory Organisation
Media
Aseptic Manipulation

BASIC ASPECTS

Cell Culture
Cellular Totipotency
Somatic Embryogenesis

APPLICATIONS TO PLANT BREEDING

Haploid Production
Triploid Production
In Vitro Pollination and Fertilization
Zygotic Embryo Culture
Somatic Hybridisation and Cybridisation
Genetic Transformation
Somaclonal and Gametoclonal Variant Selection

APPLICATION TO HORTICULTURE AND FORESTRY

Production of Disease-Free Plants
Clonal Propagation

GENERAL APPLICATIONS

Industrial Applications: Secondary Metabolite Production Germplasm Conservation

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