Applications and Protocols
edited by
B.W. Hicks
Humana Press, June, 2002, 385 pages. $99.50 + shipping ($5.00, U.S. or $15.00 Elsewhere)
Table of Contents
Part I. Manipulation of GFP Structure at the Genetic Level
Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissue To Search for New Genes
Mikhail Matz
Use of cobA and cysGA as Red Fluorescent Indicators
Charles Roessner
Circular Permutation of the Green Fluorescent Protein
Simon Topell and Rudi Glockshuber
Evolutionary Design of Generic GFP Biosensors
Nobuhide Doi and Hiroshi Yanagawa
Random Insertion of GFP into the Regulatory Subunit of cAMP Dependent Protein Kinase
Pascal Baehler, Ricardo M. Biondi, Miguel van Bemmelen, Michel Veron and Christophe Reymond
Cirular mRNA Encoding for Monomeric and Polymeric GFP
Rhonda Perriman
Part II. Detection and Imaging of GFP
Fluorescence Lifetime Imaging of Green Fluorescent Fusion Proteins in Living Cells
Ammasi Periasamy, Masilamani Elangovan, Elizabeth Elliott and David L. Brautigan
Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein: Energy Transfer to the Endogenous Chromophores of Phycobilisome Light-Harvesting Complexes
Jasper J. van Thor and Klaas J.Hellingwerf
BRET Assays for Protein-Protein Interactions in Living Cells
Yao Xu, Carl H. Johnson and David W. Piston
Whole-Body Fluorescence Imaging with GFP
Robert M. Hoffman
Part III. GFP to Monitor Protein Distribution and Trafficking
Drug-Induced Translocation of Nucleolar Proteins Fused to GFP
Benigno C. Valdez and Laszlo Perlaky
Light-Induced Nuclear Targeting of PhytochromeB-sGFP in Plants
Akira Nagatani and Tomonao Matsushita
Mechanisms of Protein Trafficking: Two Different Signal Sequences Fused to GFP to Study Mitochondrial Import
Henry Weiner
Analysis of Nucleo-Cytoplasmic Transport Using GFP
Roland Stauber
Part IV. GFP in Transgenic Organisms
Transgenic Cattle: Bovine Embryo Selection Using GFP
Anthony W.S. Chan, Kowit-Yu Chong, and Gerald Schatten
Transgenic Mice: Development of GPI-Anchored EGFP for One-Step Visualization of GFP Fate in Global
Tissues and Ubiquitous Cell Surface Marking
Gen Kondoh
Transgenic Zebrafish Expressing GFP
Ebrahim Shafizadeh, Haigen Huang and Shuo Lin
Transgenic Insects: Expressing GFP-Silk Fibroin Light Chain Fusion Protein in Transgenic Silkworms
Hajime Mori
GFP in Transgenic Plants: Brassica Transformation
C. Neal Stewart Jr., Matthew D. Halfhill, and Reginald J. Millwood
Part V. GFP Biosensors
GFP Calcium Biosensors: Calcium Imaging with GFP Cameleons
Guy Rutter
GFP "Fluobody" Immunosensors: Immunofluorescence with GFP-Antibody Fusion Proteins
Arjen Schots and Jan M. van der Wolf
Green Fluorescent Protein-Based Protein Kinase Biosensor Substrates
Scott Ulrich and Kevan Shokat
GFP Urea Biosensors: Uropathogenic Proteus mirabilis
Christopher Coker, Hui Zhao and Harry L.T. Mobley
Part VI. Viral Applications of GFP
Using GFP to Monitor Measles Virus Cell-to-Cell Spread by Time-Lapse Confocal Microscopy
W. Paul Duprex and Bert K. Rima
Tracking and Selection of Retrovirally Transduced Murine Bone Marrow Cells Using Green Fluorescent Protein
Jessamyn Bagley and John Iacommini
GFP as a Reporter of Adenovirus-Mediated Gene Transfer and Expression in the Hypothalamic
Neurohypophyseal System
Elisardo Corral Vasquez and Alan Kim Johnson
Enhancement of GFP Expression in Adeno-Associated Virus with the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element
Jonathan E. Loeb, Matthew D. Weitzman and Thomas J. Hope
Construction of Infectious Simian Varicella Virus Expressing GFP
Ravi Mahalingham and Donald H. Gilden
GFP in Retrovial Vector Constructs as Marker and Reporter of Gene Expression for Cell and Gene Therapy
Applications
Nicoletta Eliopoulos and Jacques Galipeau
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