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"Green Fluorescent Protein"

Applications and Protocols

edited by B.W. Hicks

Humana Press, June, 2002, 385 pages. $99.50 + shipping ($5.00, U.S. or $15.00 Elsewhere)

Table of Contents

Part I. Manipulation of GFP Structure at the Genetic Level

Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissue To Search for New Genes
Mikhail Matz

Use of cobA and cysGA as Red Fluorescent Indicators
Charles Roessner

Circular Permutation of the Green Fluorescent Protein
Simon Topell and Rudi Glockshuber

Evolutionary Design of Generic GFP Biosensors
Nobuhide Doi and Hiroshi Yanagawa

Random Insertion of GFP into the Regulatory Subunit of cAMP Dependent Protein Kinase
Pascal Baehler, Ricardo M. Biondi, Miguel van Bemmelen, Michel Veron and Christophe Reymond

Cirular mRNA Encoding for Monomeric and Polymeric GFP
Rhonda Perriman

Part II. Detection and Imaging of GFP

Fluorescence Lifetime Imaging of Green Fluorescent Fusion Proteins in Living Cells
Ammasi Periasamy, Masilamani Elangovan, Elizabeth Elliott and David L. Brautigan

Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein: Energy Transfer to the Endogenous Chromophores of Phycobilisome Light-Harvesting Complexes
Jasper J. van Thor and Klaas J.Hellingwerf

BRET Assays for Protein-Protein Interactions in Living Cells
Yao Xu, Carl H. Johnson and David W. Piston

Whole-Body Fluorescence Imaging with GFP
Robert M. Hoffman

Part III. GFP to Monitor Protein Distribution and Trafficking

Drug-Induced Translocation of Nucleolar Proteins Fused to GFP
Benigno C. Valdez and Laszlo Perlaky

Light-Induced Nuclear Targeting of PhytochromeB-sGFP in Plants
Akira Nagatani and Tomonao Matsushita

Mechanisms of Protein Trafficking: Two Different Signal Sequences Fused to GFP to Study Mitochondrial Import
Henry Weiner

Analysis of Nucleo-Cytoplasmic Transport Using GFP
Roland Stauber

Part IV. GFP in Transgenic Organisms

Transgenic Cattle: Bovine Embryo Selection Using GFP
Anthony W.S. Chan, Kowit-Yu Chong, and Gerald Schatten

Transgenic Mice: Development of GPI-Anchored EGFP for One-Step Visualization of GFP Fate in Global
Tissues and Ubiquitous Cell Surface Marking Gen Kondoh

Transgenic Zebrafish Expressing GFP
Ebrahim Shafizadeh, Haigen Huang and Shuo Lin

Transgenic Insects: Expressing GFP-Silk Fibroin Light Chain Fusion Protein in Transgenic Silkworms
Hajime Mori

GFP in Transgenic Plants: Brassica Transformation
C. Neal Stewart Jr., Matthew D. Halfhill, and Reginald J. Millwood

Part V. GFP Biosensors

GFP Calcium Biosensors: Calcium Imaging with GFP Cameleons
Guy Rutter

GFP "Fluobody" Immunosensors: Immunofluorescence with GFP-Antibody Fusion Proteins
Arjen Schots and Jan M. van der Wolf

Green Fluorescent Protein-Based Protein Kinase Biosensor Substrates
Scott Ulrich and Kevan Shokat

GFP Urea Biosensors: Uropathogenic Proteus mirabilis
Christopher Coker, Hui Zhao and Harry L.T. Mobley

Part VI. Viral Applications of GFP

Using GFP to Monitor Measles Virus Cell-to-Cell Spread by Time-Lapse Confocal Microscopy
W. Paul Duprex and Bert K. Rima

Tracking and Selection of Retrovirally Transduced Murine Bone Marrow Cells Using Green Fluorescent Protein
Jessamyn Bagley and John Iacommini

GFP as a Reporter of Adenovirus-Mediated Gene Transfer and Expression in the Hypothalamic
Neurohypophyseal System Elisardo Corral Vasquez and Alan Kim Johnson

Enhancement of GFP Expression in Adeno-Associated Virus with the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element
Jonathan E. Loeb, Matthew D. Weitzman and Thomas J. Hope

Construction of Infectious Simian Varicella Virus Expressing GFP
Ravi Mahalingham and Donald H. Gilden

GFP in Retrovial Vector Constructs as Marker and Reporter of Gene Expression for Cell and Gene Therapy
Applications Nicoletta Eliopoulos and Jacques Galipeau

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