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"Cereal Genomics"

Methods and Protocols

edited by Henry, Robert J.; Furtado, Agnelo

2014, X, 300 p., 39 illus., 29 illus. in color,Hardcover
Price: $119.00 (Shipping and handling: $8.00 for U.S. delivery, $20.00 elsewhere).

CONTENTS

1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing

2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)

3. RNA Extraction from Cereal Vegetative Tissue

4. RNA Extraction from Developing or Mature Wheat Seeds

5. cDNA Library Preparation

6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants

7. The Polymerase Chain Reaction (PCR) - General Methods

8. Mutation and Mutation Screening

9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression

10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel

11. Rapid Cloning of Genes and Promoters for Functional Analyses

12. Genome Walking

13. Functional Analysis by Protein Biochemistry

14. Genomic Southern Blot Analysis

15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs

16. Protein Blotting-Protocol for Beginners

17. Genetic Transformation of Wheat via Particle Bombardment

18. Sorghum Genetic Transformation by Particle Bombardment

19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery

20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation

21. Agrobacterium-mediated Transformation- Rice Transformation

22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos

23. A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops

24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data

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Agritech Publications/Agricell Report

P.O. Box 255
Shrub Oak, NY 10588, U.S.A.
Phone/Fax: (914) 528-3469
E-mail: Agritech@AgritechPublications.com