P.O. BOX 255
SHRUB OAK, NY, 10588, U.S.A.
FAX/PHONE: 914 528 3469
E-MAIL: Agritech@AgritechPublications.com


"Plant Tissue Culture Case Histories " CD

by Ronald A. de Fossard

CD only, 2008 edition with excel spreadsheet. Price: $89.00 + shipping ($1.50, U.S. or $4.50 Elsewhere)

What is on this CD?

  • "Case Histories" section (PDF)
  • "Photographs of Species TC-Propagated" (PDF)
  • "Culture Media" section (PDF)
  • "Experimentations" section (PDF)
  • "Statistical Analysis of Experimental Data" section (PDF)
  • Interactive Spreadsheets (Excel)

Recommended Computer System Requirements:

  • Windows 2000/XP operating system or higher
  • 1024 x 768 display resolution
  • Adobe Acrobat Reader v7 or higher
  • Javascript enabled web browser

 

PLANT TISSUE CULTURE CASE HISTORIES
Publication: Table of Contents

 

CASE HISTORIES FORMAT (1976)

 

SEEDS

  • Aseptic Seed Germination and Seedling Growth

FERNS

  • Fern Callus Formation and Differentiation into GAMETOPHYTIC or Sporophytic Tissues
  • Propagation of Ferns by Spores in Sterile Culture
  • The Induction of Apogamy in the Bracken Fern

DICOTS

  • ACTINIDIACEAE
    • Organ Culture for the Propagation of Chinese Gooseberry
      (Actinidia chinensis PL.)

  • AIZOACEAE
    • Plants from Mesembryanthemum floribundum Callus

  • BEGONIACEAE
    • Propagation of Begonia x cheimantha from Petiole Segments

  • CARYOPHYLLACEAE
    • Carnation Shoot Tip Culture
    • Multiplication of Carnations from Shoot Apices

  • COMPOSITAE
    • Chrysanthemum Shoot Tip Culture
    • Rapid Multiplication of Gerbera jamesonii Bolus
    • Vegetative Propagation of Pyrethrum and Other Composites by Capitulum Culture

  • CONVOLVULACEAE
    • Tip Culture of Sweet Potato (Ipomoea batatus (L.) LAM.)

  • CRUCIFERAE
    • PlantsPropagation of Brussels Sprouts (Brassica oleraceae L. var. gemmiera DC.)
    • Cauliflower Plants from Curds by "Meristem" Culture

  • EUPHORBIACEAE
    • Stem Tip Culture of Cassava, Manihot esculentus Crantz (M.utillissimus POHL.)

  • GERANIACEAE
    • Geranium (Pelargonium) Plants from Callus

  • GESNERIACEAE
    • Multiplication of Gloxinias by Culture of Peduncles

  • GROSSULARIACEAE
    • Gooseberry Propagation by Stem Tip Culture

  • LABIATAE
    • Apical Meristem Culture of Coleus

  • EUPHORBIACEAE
    • Stem Tip Culture of Cassava, Manihot esculentus Crantz (M.utillissimus POHL.)

  • GERANIACEAE
    • Geranium (Pelargonium) Plants from Callus

  • GESNERIACEAE
    • Multiplication of Gloxinias by Culture of Peduncles

  • GROSSULARIACEAE
    • Gooseberry Propagation by Stem Tip Culture

  • LABIATAE
    • Apical Meristem Culture of Coleus

  • LEGUMINOSAE
    • Plant Regeneration from Callus of Stylosanthes hamata SW.
    • Plants from Callus of Pea (pisum sativum L.)
    • Regeneration of Alfalfa (Medicago sativa L.) from Callus

  • MYRTACEAE
    • Clonal Propagation of Eucalyptus grandis L. by Nodal Culture

  • PEPEROMIACEAE
    • Regeneration from Leaf Squares of Peperomia Sandersii A.DC.

  • POLYGONACEAE
    • Propagation of Rhubarb (Rheum rhaponticum L.) by Stem Tip Culture

  • RANUNCULACEAE
    • Embryo Formation in Liquid Cultures of Nigella damascena L.

  • ROSACEAE
    • Multiplication of Apple Plants from Shoot TIp Cultures
    • Plant Regeneration from Almond Callus
    • Stem Tip Culture of Rosa multiflora THUNB.

  • RUTACEAE
    • Plants from the Nucellus of Several Species of Citrus
    • Propagation of Citrus madurensis L. from Internodal Segments

  • SALICACEAE
    • Plant Formation from Callus of Quaking Aspen (Populus tremuloides MICHX)

  • SOLANACEAE
    • Bud Formation and Embryo Differentiation from Petunia Cultures
    • Regeneration of Shoots and Plants from Tobacco Pith Callus
    • Stem Tip Culture of Potato (Solanum tuberosum L.)

  • ULMACEAE
    • Propagation of Elm (Ulmus americana) from Cell Suspension Culture

  • UMBELLIFERAE
    • Clona lCarrot Plants from Suspension Cultures

  • VITIDACEAE
    • Callus and Cell Culture from Grape Berries (Vitis vinifera L.)

MONOCOTS

  • AMARYLLIDACEAE
    • Bud and Root Formation from Excised Bulb Scales of Hippeastrum hybridum

  • ARACEAE
    • Plant Formation in Callus Tissues of Anthurium andraeanum LIND

  • GRAMINEAE
    • Meristem Culture of Lolium multiflorum LAM.
    • Plant Differentiation from Maize (Zea mays L.) Callus
    • Plant Differentiation from Sugarcane (Saccharum) Callus. I.
    • Plant Differentiation from Sugarcane (Saccharum) Callus. II.

  • IRIDACEAE
    • Gladiolus Plants from Callus Cultures
    • Propagation of Dutch Iris (Iris hollandica) by Tissue Culture
    • Vegetative Propagation of Freesia Through Callus Culture

  • LILIACEAE
    • Bulblet Regeneration of Hyacinthus orientalis L.
    • Plant Regeneration from Suspension Cultures of Lilium
    • Rapid Vegatative Propagation of Asparagus through Lateral Bud Culture

  • MUSACEAE
    • Banana Plants from Meristem Culture

 

ORCHIDS

  • Professor Yoneo Sagawa's Methods for Orchids
  • Growth and Development of Unripe Seeds of Cattleya
  • Clonal Propagation of Cattleya Orchids
  • Clonal Propagation of Cymbidium Orchids
  • Laeliocattleya Propagation by Leaf Tip Culture
  • Broad Spectrum Experimentation with Cymbidium

CONIFERS

  • PINACEAE
    • Broad Spectrum Media for Pinus radiata Don. Embryo Culture
    • Adventitious Bud Formation from Douglas Fir Callus
    • Differentiation of Buds on Cultured Cotyledons of Pinus palustris MILL.

HAPLOID CULTURES

  • GERANIACEAE
    • Prooduction of Haploid Sporophytes from Perlargonium hortorum Bailey

  • GESNERIACEAE
    • Haploid Sporophytes of African Violet (Saintpaulia ionantha H.Wendl.)

  • GRAMINEAE
    • Haploid Sporophytes from Wehat Pollen on a Potato Medium

  • SOLANACEAE
    • Tobacco Haploid Plants (Sporophytes) from Anther Culture

-----------------------------------------------------------------------------------

 

PHOTOGRAPHS of SPECIES TC-PROPAGATED in my LABORATORY

 

 

Introduction

TISSUE CULTURE PROPAGATION of Aroids

Tissue culture propagation of cordylines

Tissue culture propagation of begonia & episcia

tissue culture propagation of ferns

tissue culture propagation of capers (Capparis spinosa)

cloning paulownia - the only woody scrophulariaceae species

tissue culture of eucalyptus

tissue culture propagation of dionaea, sarracenia and nepenthes

cloning of scaevola aemula (goodeniaceae)

cloning of neomarica - the walking iris (iridaceae)

 

-----------------------------------------------------------------------------------

 

PLANT TISSUE CULTURE - CULTURE MEDIA

 

WELL KNOWN PUBLISHED TISSUE CULTURE MEDIA

  • Chemical Compounds (mass and mole amounts), Preparation of x100
  • Concentrated Stock Solutions and Amounts per Litre of Medium
  • WHITE'S (1943) MEDIUM White, P.R.,
  • NITSCH'S (1951) MEDIUM Nitsch,.J.P.,
  • HILDEBRANDT'S SUNFLOWER MEDIUM (1962) Hildebrandt, A.C.,
  • HILDEBRANDT'S TOBACCO MEDIUM (1962) Hildebrandt,.A.C.,
  • MURASHIGE & SKOOG MEDIUM (1962) Murashige, T., and Skoog, F.,
  • BLAYDES' (1966) MEDIUM Blaydes,D.F.,
  • B5-MEDIUM (1968) Gambord,O.L., Miller,R.A. and Ojima.K,.
  • NITSCH & NITSCH'S (1969) MEDIUM Nitsch,.J.P., and Nitsch,C.,
  • SCHENK & HILDEBRANDT'S (1972) MEDIUM Schenk,R.U., and HILDEBRANDT,A.C.
  • WOODY PLANT MEDIUM (1980) Lloyd,G., and McCown,B.,
  • DKW MEDIUM (1984) Driver,.J.A., and Kuniyuki,A.H.
  • KNOP'S MINERALS (1865) Knop,W.,
  • KNUDSON'S MEDIUM (1946) C Knudson,L.
  • HELLER'S MEDIUM (1953) Heller,R.,
  • VACIN and WENT'S MEDIUM (1949) Vacin,E., and Went,F.,
  • MOREL and MULLER'S MEDIUM (1964) Morel,G., & Muller,J.F.,
  • WIMBER'S MEDIUM (1963) Wimber, D.E.,

 

TABLE of COMPOUNDS & CONCENTRATED CHEMICAL STOCK SOLUTION S for "TIDIED-UP" MEDIA

  • (a) MACRONUTRIENT ELEMENTS
  • (b) MICRONUTRIENT ELEMENTS
  • (c) MICRONUTRIENT ELEMENTS
  • (d) MAIN CARBON SOURCE
  • (e) PLANT GROWTH REGULATORS
    • AUXINS
    • CYTOKININS
    GIBBERELLINS
  • (f) OTHER GROWTH FACTORS
  • (g) AMINO ACIDS
  • (h) UNDEFINED SUBSTANCES
  • (i) OTHER SUBSTANCES

WELL-KNOWN PUBLISHED TISSUE CULTURE MEDIA: "TIDIED-UP" VERSIONS

  • WHITE'S (1943) MEDIUM
  • MURASHIGE & SKOOG (1962) MEDIUM
  • SCHENK & HILDEBRANDT'S (1972)
  • GAMBORG, MILLER & OJIMA'S (1968) B5-MEDIUM
  • LLOYD,G., and McCOWN,B.'s-WPM's (1980)
  • NITSCH'S (1951) MEDIUM
  • NITSCH & NITSCH'S (1969) MEDIUM
  • DKW MEDIUM (1984)
  • KNOP (1865) MEDIUM
  • KNUDSON(1946) Medium
  • VACIN & WENT (1949) MEDIUM
  • MOREL & MULLER (1964) MEDIUM
  • WIMBER (1963) MEDIUM
  • HELLER (1953) MEDIUM

PROPOSED CASE HISTORY FORMAT

  • TITLE:
  • EXPLANT:
  • DISINFESTATION:
  • CULTURE MEDIUM:
  • (1) PHYSICAL CONDITIONS:
  • (2) CHEMICAL CONSTITUTION:
  • INCUBATION:
  • COMMENTS:
  • REFERENCES:
  • CONTRIBUTED BY:-

 

PREPARATION OF CONCENTRATED CHEMICAL STOCK SOLUTIONS FOR BROAD SPECTRUM CULTURE MEDIA

  • INTRODUCTION
  • BROAD SPECTRUM CULTURE MEDIA: CODING SYSTEM
  • BROAD SPECTRUM CONCENTRATED CHEMICAL STOCK SOLUTIONS
  • CONCENTRATION FACTORS (CF) IN BROAD SPECTRUM CULTURE MEDIUM
  • PREPARATION of STOCK SOLUTIONS
  • MINERAL STOCK SOLUTIONS
  • AUXIN STOCK SOLUTIONS
  • CYTOKININ STOCK SOLUTIONS
  • GROWTH FACTOR STOCK SOLUTIONS

BROAD SPECTRUM CULTURE MEDIA in CHRONOLOGICAL ORDER

  • CODE LETTERS CLOSED

BROAD SPECTRUM CULTURE MEDIA in CHRONOLOGICAL ORDER

  • CODE LETTERS EXPANDED

BROAD SPECTRUM CULTURE MEDIA REARRANGED for analysis

  • CODE LETTERS EXPANDED

PREPARATION of CULTURE MEDIA

  • ORGANISATION and FORMAT of the SPREADSHEET FILE USED by the AUTHOR for the PREPARATION of CULTURE MEDIA
  • Preparation of MEDIUM-1
  • Preparation of MEDIUM-4
  • Preparation of MEDIUM-7
  • Preparation of MEDIUM-8
  • Preparation of MEDIUM-9
  • Preparation of MEDIUM-10
  • Preparation of MEDIUM-11
  • Preparation of MEDIUM-14
  • Preparation of MEDIUM-16
  • Preparation of MEDIUM-17
  • Preparation of MEDIUM-18
  • Preparation of MEDIUM-19
  • Preparation of MEDIUM-32
  • Preparation of MEDIUM-43
  • Preparation of MEDIUM-55
  • Preparation of MEDIUM-68
  • Preparation of MEDIUM-70
  • Preparation of MEDIUM-88
  • Preparation of MEDIUM-89
  • Preparation of MEDIUM-93
  • Preparation of MEDIUM-97
  • Preparation of MEDIUM-99
  • Preparation of MEDIUM-100
  • Preparation of MEDIUM-101
  • Preparation of MEDIUM-105
  • Preparation of MEDIUM-107
  • Preparation of MEDIUM-108
  • Preparation of MEDIUM-110

 

ANALYSIS OF PUBLished culture media

  • OBJECTIVE: To translate published culture media into a form allowing comparisons to be made with other media.
  • Table 1 Compounds frequently used in different culture media
  • Table 2 Template for Translating Different Culture Media
    • (1) WOODY PLANT MEDIUM Lloyd,G. and McCown,B.,
    • (2) de Fossard's Medium-1

 

-----------------------------------------------------------------------------------

 

PLANT TISSUE CULTURE - EXPERIMENTATIONS

 

STEP by step procedures to acheive clonal propagation of a cultivar

From my CD Plant Tissue Culture Propagation (2007; pages 91-93)

  • INTRODUCTION
  • PATHWAY-I: EXPERIMENTATION WITH ASEPTIC SEEDLINGS
  • PATHWAY-II: INITIAL CULTURE AND GROWTH OF SELECTED GENOTYPE

CHAPTER 5 THE BROAD SPECTRUM APPROACH to MEDIA

From my CD Commercial Micropropagation (2007; pages 87-98))

  • INTRODUCTION
  • STANDARDIZED CODING OF BROAD SPECTRUM MEDIA
  • Table 5.1 Constituents and Concentration sof the 1974 Broad Specturm Experiment
  • Table 5.2 Four-Letter Code describing Broad Spectrum Culture Media
  • THE TOBACCO BROAD SPECTRUM EXPERIMENT (1974)
  • THE EUCALYPTUS BROAD SPECTRUM EXPERIMENT (1970's)
  • THE BROAD SPECTRUM EXPERIMENT IN THE 1980s
  • Table 5.3 Basal medium consisting of micronutrients, growth factors and gelling agent.
  • Table 5.4 Full strength (x1) macronutrients and iron comopound.
  • SUGGESTED INITIAL EXPERIMENTS
  • Table 5.5 Selecton of a Suitable Culture Medium for Organ Culture
  • EXAMINING BROAD SPECTRUM EXPERIMENTS
  • Table 5.6 Format for Recording Results and Experimental Details from a Mini Broad Spectrum Experiment
  • Table 5.7 Omission Type Experiments
  • Table 5.8 Follow of Omission Type Experiment
  • Table 5.9 Dose Response Experiments
  • COMMERCIAL MICROPROPAGATION

 

APPENDIX #:- EXPERIMENTATION

From my CD Commercial Micropropagation (2007)

  • INTRODUCTION
  • DEFINITION OF OBJECTIVES OF EXPERIMENTS
  • MODELS OF EXPERIMENTS
  • A Mini Broad Spectrum Experiment
    • Planning the Experiment
    • Preparation Details
  • A Omission Experiments
    • Preparation Details of Omission Experiments
    • A. Auxin Experiment (8 treatments)
    • B. Cytokinin Experiment (7 treatments)
    • C. Minerals Experiment (3 treatments)
    • D. Sucrose Experiment (3 treatments)
  • Dose Response Experiments
    • Preparation of IAA Dose Response Experiment
    • Preparation of BAP Dose Response Experiment
    • Preparation of Sucrose Dose Response Experiment

FINDING SUITABLE CULTURE MEDIA

From my CD Plant Tissue Culture Propagation (2007 Update; pages 9-18)

  • INTRODUCTION
  • BROAD SPECTRUM EXPERIMENTATION
  • EXPLORATORY EXPERIMENT-I
    • Objective
    • Basal Medium
    • Treatments
    • Preparaton Details for Basal Medium Components
  • EXPLORATORY EXPERIMENT-II
    • Objective
    • Basal Medium
    • Treatments
    • Strategy for Preparation of Treatments

 

-----------------------------------------------------------------------------------

 

STATISTICAL ANALYSIS of EXPERIMENTAL DATA

 

ABSTRACT

Preface

BIOASSAY FOR AUXINS a CLass experiment from the 1970s

Introduction

procedure

  • Preparaton of wheat seedlings
  • Treatments
  • Preparation of Stock Solutions
  • Preparation of Petri dishes and treatments
    • Table 1: SCHEDULE for PREPARATION of TREATMENT PETRI DISHES
  • Replicates
  • Incubation
  • Results
    • Table 2: LENGTH (to nearest mm) of SEGMENTS AFTER INCUBATION
    • Table 3: Re-arranging treatments for first ANOVA analysis involving 20 treatments; Isolation of Treatments not involved in first ANOVA analysis;

     

ANOVA: TWO-FACTOR WITHOUT REPLICATION

  • Table 3: Re-arranging treatments for first ANOVA analysis involving 20 treatments
  • SUMMARY
  • ANOVA
  • Least Significant Differences (LSDs)
  • Table and Histogram
  • Interpretation of First Anova Analysis
  • Summary
  • Treatments

ANOVA: TWO-FACTOR WITH REPLICATION

  • Re-arranging treatments for second ANOVA analysis involving 16 treatments, eight IAA and two Basal Media
  • SUMMARY
  • ANOVA
  • Least Significant Differences (LSDs)
  • Table and Histogram

COMPARING EXCEL's ANOVA with The University of New England's AOV

  1. ANOVA (EXCEL) table: 5% Probablility Level
  2. AOV (UNE) table
  3. ANOVA (EXCEL) table: 1% Probability Level
  4. ANOVA (EXCEL) table: 0.1% Probability Level
  5. Least Significant Differences (LSDs)
  6. Table of Means with 5%, 1% and 0.1% LSD
  7. Least Significant Differences (LSDs)
    1. BASAL MEDIUM
    2. IAA DOSE-RESPONSE
    3. IAA x BASAL MEDIA INTERACTION
  8. NOTE FOR THESIS PREPARATION
  9. Sorted MEans
  10. Sorted Studentized Range

MULTIPLE REGRESSION ANALYSIS (MRA)

 

MRA: 2nd order polymonial involving 10 treatments

 

SUMMARY output & anova BM1 & bm2 and Equations

 

Equations & Derived values

 

Graphs

 

MRA: Linear equation involving 10 treatments

 

SUMMARY, ANOVA, EQUATION for BM1

SUMMARY, ANOVA, EQUATION for BM2


DERIVED VALUES

 


GRAPH

 

CALCULUS

  • Using Calculus to Estimate Optimal IAA Concentrations
  • Comparing Excel's MRA with that of THE UNIVERSITY of NEW ENGLAND

A FEW DEFINITIONS

 

ESTIMATION OF "UNKNOWNS": Solving Quadrative Equations from Regression Equatons

 

APPENDIX I: Calculation of Mean, Standard Deviation, Standard Error and Orefficient of Variation

 

APPENDIX II: TRANSFORMATIONS

 

APPENDIX III: Student's t test

 

APPENDIX IV: Table of F-Statistics p=0.05, p=0.01 and p=0.001

 



REPRODUCED from my CD Commercial Micropropagation


APPENDIX D: Recoreds, Experimental Desings and Statistical Analysis of Data

  • Experimental Design and Statistical Analyses of Data
  • Analysis of Variance and Studentized Range Method
  • Equal Increment Dose-Response Experiments
  • Transformation of Units of Molarity for Computation in Multiple Regression Analysis
  • The X2 Test (the Chi2 Test)
  • Number of Replicates in Plant Tissue Culture Work
    • Example 1: Sunflower Anther Callus
    • Example 2: Anther Culture of Cereals
  • Calculation of the Number of Replicates
  • Replication in Equal Increment Dose-Response Type Experiments
  • Calculation of Mean, Standard Deviation , Standard Error and Coefficient of Variation
  • Transformation of Data