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"Plant Tissue Culture Propagation CD"

1993 edition CD with 2006 update

by Ronald A. de Fossard

CD only, 197 pp + 98 pp, 872 fully restored color photographs in 218 slides. $89.00 + shipping ($1.50, U.S. or $4.50 Elsewhere)


Preparation Area

Preparation of Concentrated Chemical Stock Solutions, Preparation of Culture
Media, Alternative Methods of Sterilizing Culture Media, Membrane Sterilization,
Sterilization with Organic Solvents, Some Other Substances Added to Culture Media,
Powdered Culture Media, Inoculation Area

Inoculation Techniques: Aseptic Procedures, Fire Hazard, Incubation Area

Disinfestation: Physical Methods, Chemical Methods, Summary of Standard Disinfestation Treatment, Recovering Cultures Contaminated with Microbes, Mini Broad Spectrum Experiment-I, Mini Broad Spectrum Experiment-II

Step by Step Procedures to Achieve Clonal Propagation of a Cultivar
Pathway-I: Experimentation with Aseptic Seedlings, Pathway-II: Initial Culture and Growth of Selected Genotype, Clonal Propagation of Strawberry

Eucalyptus Research
Eucalyptus Callus
Pathway-I:- Organ Culture with Eucalyptus Seedling Material
Pathway-II : - Or gan Culture with Adult Eucalyptus Trees
Present Direction of Eucalyptus Research
Eriostemon Research

Initiation of Tissue Cultures of a Range of Plant Types
Dioscorea Discolor (Dioscoreaceae), Jewel Orchid (Haemaria Discolor X "Dawsoniana") (Orchidaceae), Saxifraga Sarmentosa (Saxifragacea), Syngonium Podophyllum (Araceae), Maranta Leuconeura, Erythrophylla (Marantaceae), Jojoba (Simmondsia Chinensis) (Simmondsiaceae), Hyacinthus Orientalis (Liliaceae), African Violet (Saintpaulia Ionantha) (Gesneriaceae), Begonia Rex-Culturum (Begoniaceae), Tissue Culture Propagation of Grevillea

Tissue Cultures of a Number of Species

Cauliflower (Brassica oleracea var. botrytis) (Cruciferae)
Hoop Pine (Araucaria cunninghamii) (Araucariaceae)
Miscellaneous Cultures
Orchid Cultures: Immature Seed Culture of a Sympodial Orchid, Initiation of Clonal Propagation of a Cymbidium, Initiation of Clonal Propagation of a Lealiocattleya, Broad Spectrum Experi mentat ion with Cymbidium Cultivars, Induction of Polyploidy with Colchicine, Protocol for Chromosome Counting, Measuring Guard Cells to Detect Polyploidy, Tissue Cultured Orchid Plants Established in Potting Mix
Ferns: Aseptic Culture of Fern Spores, Clonal Propagation of Nepholepis Ferns, Planting out of Tissue Cultured Plants
Defining the Problems Associated with Planting Out: Desiccation, Microbial Contamination, Photosynthesis, Rooting, Production Costs of Tissue Cultured Plants
Virus Eradication: Strategy to Eradicate Viruses by a Combination of Heat Treatment and Meristem Culture

Tobacco Callus Research
Anther Culture and Haploid Sporophytes: Tobacco Anther Culture: Rice Anther Culture, Sunflower Anther Culture
Bibliography, Index, About the Author

2007 addition - Table of Contents
Plant Tissue Culture Propagation 2007
iii + 95 + 2 plates + 35 color photographs


Layout of Laboratory: Entrace and Hallwa y, Off ice/Study, Preparation Area, Preparation of Concentrated Chemical Stock Solutions, Cold Dispensing of Culture Medium, Inoculation Room and Laminar Flow Layout, Incubation Rooms, Lights and Shelving for Cultures, Which Species are incubated in Each Room? Now for a Really Large Scale Micropropagation Business, Finding Suitable Culture Media

Broad Spectrum Experimentation
Exploratory Experiment-.I Objective: To establish the most promising concentrations of minerals and sucrose for a species so as to have a good Basal medium for later experimentation.

Exploratory Experiment-II. Objective: To establish the most promising concentrations of auxins and sytokinins for a species using the new selected Basal Medium from EXPLORATORY EXPERIMENT-I


Working Hypothesis No.1: Contamination is caused by poor technique, by poor hygiene in the laboratory or by faulty autoclaving.

Working Hypothesis No.2: Population of micro bes is too large to be controlled by prophylactic control methods, Development of Disinfection Protocols, Devleopment of Disinfection Protocols for Late Contamination, Conclusion

Working Hypothesis No.3: Chlorine treatments are capable of killing all microbes except under certain conditions. Disinfestation with Pool Chlorine An Iodometric Method for Measuring Available Chlorine, Content of Hypochlorite Solutions, Reagents, Procedure to Estimate Available Chlorine Content of a Solution, Calculation of Available chlorine Concentration, in Test Solution, Note from PLANT-TC LIST (17 February 1999)

Working Hypothesis No.4: Contamination of cultures, after numerous passages, is either due to long hidden microbes (low initial population and/or unsuitability of culture medium) or to the isolation and nurturing of chlorine-resistant strains of microbes. Summary of Possible Mechanisms of Drug-Resistance, Resistance to Penicillin

Working Hypothesis N o.5: P lants in culture produce antibiotics capable of inhibiting the growth of microbes.

Working Hypothesis No.6: The role of ingredients in culture media relative to microbial contamination: Sucrose, Salt, Ingredients in media used for micropropagation

Working Hypothesis No.7: There is an inbuilt destiny to cultures that determines their longevity. After a predetermined number of passages their growth declines, they aged and in consequence, are at the mercy of many types of hazards.

Concluding Working Hypothesis: Currently we have a four-pronged strategy A review of Some Pertinent Literature


What are mites?
The Pure Science Approach to Problems with Pests, Prophylactic suggestions from the Factory (Laboratory) floor. What do we do with Mites in our Laboratory? Final Words, Major Research Work

Post-Harvest Physiological Disorder of Oranges
Low Volume Spraying, Citrus Viruses

The Development of New Techniques and Methods:

Glass and Plastic Culture Vessels, The Addition of Sterilized Chemicals and Plant Extracts to Autoclaved Culture Media, Home-made Tungsten Wire Needles for Fine Dissections such as Apical Meristems, "Sausages" for the Incubation of Cultures, Analysis of Culture Media and Use of Mole Units, The Broad Spectrum Tissue Culture Experiment, Cloning of Eucalyptus Using Tissue Culture, Haploid Plants, Histochemistry, Blue-Green Algae, Non-Botanical Activities, Text Books on Clonal Micropropagation, Sundry Publications, Bibliography

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