"Arabidopsis Protocols"

Centro de Investigacion y Technologia, Madrid, Spain

Published: 1997. Hardcover. 472 pp.

Price $109.50

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Internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work with Arabidopsis genetics and molecular biology. These time-tested methods range from techniques for the successful growing of Arabidopsis to strategies for gene cloning. The sections on genetics, transformation, and gene expression analysis will be especially helpful to scientists involved in mutant analysis or in producing and analyzing transgenic plants. In many cases, the protocols can also be applied to other plant species, with minor adjustments. Serving both experienced researchers and beginners in the field, Arabidopsis Protocols will immediately become the standard reference for laboratories working with Arabidopsis, the plant of choice to approach many fundamental questions in plant biology.

Contents:

Part I. Arabidopsis Culture. Growth of Plants and Preservation of Seeds, Randy Scholl, Luz Rivero-Lepinckas, and Deborah Crist. Sterile Techniques in Arabidopsis, Peter McCourt and Kallie Keith. Control of Pests and Diseases of Arabidopsis, Mary Anderson. Establishment and Maintenance of Cell Suspension Cultures, Jaideep Mathur and Csaba Koncz. Callus Culture and Regeneration, Jaideep Mathur and Csaba Koncz. Protoplast Isolation, Culture, and Regeneration, Jaideep Mathur and Csaba Koncz. Part II. Purification of Subcellular Organelles and Macromolecules. Preparation of Physiologically Active Chloroplasts from Arabidopsis, Ljerka Kunst. Purification of Mitochondria from Arabidopsis, Mathieu Klein, Stefan Binder, and Axel Brennicke. Preparation of DNA from Arabidopsis, Jianming Li and Joanne Chory. High Molecular Weight DNA Extraction from Arabidopsis, David Bouchez and Christine Camilleri . Chloroplast DNA Isolation, George S. Mourad . Purification of Mitochondrial DNA from Green Tissues of Arabidopsis, Mathieu Klein, Rudolf Hiesel, Charles André, and Axel Brennicke. Preparation of RNA, Clifford D. Carpenter and Anne E. Simon. Part III.,Mutagenesis and Genetic Analysis. Seed Mutagenesis of Arabidopsis, Jonathan Lightner and Timothy Caspar. Genetic Analysis, Maarten Koornneef, Carlos Alonso-Blanco, and Piet Stam. Cytogenetic Analysis of Arabidopsis, John S. Heslop-Harrison. PCR-Based Identification of T-DNA Insertion Mutants, Rodney G. Winkler and Kenneth A. Feldmann. Part IV.,Gene Mapping in Arabidopsis. The Use of Recombinant Inbred Lines (RILs) for Genetic Mapping, Carlos Alonso-Blanco, Maarten Koornneef, and Piet Stam. AFLPTM Fingerprinting of Arabidopsis, Pieter Vos. Building a High-Density Genetic Map Using the AFLPTM Technology, Martin T. R. Kuiper. Use of Cleaved Amplified Polymorphic Sequences (CAPS) as Genetic Markers in Arabidopsis thaliana, Jane Glazebrook Eliana Drenkard, Daphne Preuss, and Frederick Ausubel. Mapping Mutations with ARMS, Anton R. Schäffner. Mapping Cloned Sequences on YACs, Francis D. Agyare, Gus Lagos, Deval Lashkari, Ronald W. Davis, and Bertrand Lemieux. Part V.,Transient and Stable Transformation. Transient Gene Expression in Protoplasts of Arabidopsis thaliana, Steffen Abel and Athanasios Theologis. Transient Expression of Foreign Genes in Tissues of Arabidopsis thaliana by Bombardment-Mediated Transformation, Motoaki Seki, Asako Iida, and Hiromichi Morikawa. Root Transformation by Agrobacterium tumefaciens, Annette C. Vergunst, Ellen C. de Waal, and Paul J. J. Hooykaas . Transformation of Arabidopsis thaliana C Leaf Discs by Agrobacterium tumefaciens, Eric van der Graaf and Paul J. J. Hooykas . In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration, Nicole Bechtold and Georges Pelletier. PEG-Mediated Protoplast Transformation with Naked DNA, Jaideep Mathur and Csaba Koncz. Part VI. Gene Cloning Strategies. Cloning Genes of Arabidopsis thaliana by Chromosome Walking, Jeffrey Leung and Jérôme Giraudat. Chromosome Landing Using an AFLPTM-Based Strategy, Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, and Maria Teresa Cervera. Transposon Tagging with Ac/Ds in Arabidopsis, Deborah Long and George Coupland . Transposon Tagging with the EN-I System, Andy Pereira and Mark G. M. Aarts. Cloning Genes from T-DNA Tagged Mutants, Brian P. Dilkes and Kenneth A. Feldmann . Part VII.,Gene Expression Analyses. In Situ Hybridization, Gary N. Drews. Whole-Mount In Situ Hybridization in Plants, Janice de Almeida Engler, Marc Van Montagu, and Gilbert Engler. Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells, William H. R. Langridge and Aladar A. Szalay . b-Glucoronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants, Marc De Block and Mieke Van Lijsebettens. In Situ Hybridization to RNA in Whole Arabidopsis Plants, Qingzhong Kong and Anne E. Simon. In Vivo Footprinting in Arabidopsis, Anna-Lisa Paul and Robert J. Ferl. Index.

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